From Waste Biomass to Cellulosic Ethanol by Separate Hydrolysis and Fermentation (SHF) with Trichoderma viride

Advanced biofuels can reduce fossil fuel use and the number of harmful compounds released during combustion, by reducing fuels. Lignocellulosic materials, especially waste biomass, are suitable substrates for production advanced biofuels. Among most expensive steps in ethanol is enzyme-based hydrolysis. Using microorganisms these costs. This study investigated effectiveness hydrolyzing three lignocellulosic biomass materials (barley straw, oak shavings, spent grains) into ethanol, after biological pretreatment with Trichoderma viride fungi. The fermentable sugars obtained from each substrate was subjected to preliminary study, correlation between temperature fungal activity decomposition determined. Ethanol produced separate hydrolysis fermentation (SHF) method. It found that not all presence T. viride. Regardless process temperature, average enzymatic fungi (activity index) ranged 1.25 1.31. 94 mL distillate, a 65% (v/v) concentration barley straw.